中子散射表明细胞色素b5深深嵌入脂质双层中。
Neutron scattering shows that cytochrome b5 penetrates deeply into the lipid bilayer.
作者信息
Gogol E P, Engelman D M
出版信息
Biophys J. 1984 Oct;46(4):491-5. doi: 10.1016/S0006-3495(84)84046-1.
Abstract
Cytochrome b5 was asymmetrically reconstituted into small lipid vesicles made of a highly deuterated phospholipid. Small-angle neutron diffraction patterns were collected in a series of H2O-D2O mixtures from vesicles consisting of lipid and native or trypsinized cytochrome b5. The second moment of the radial distribution of scattering density in the vesicles was derived from these data and was compared to values calculated from three proposed models, which differ by the degree that cytochrome b5 penetrates the lipid bilayer. The model in which the hydrophobic domain of the protein is distributed across the bilayer agreed most closely with the data.
摘要
细胞色素b5被不对称地重构成由高度氘代磷脂制成的小脂质囊泡。在一系列由脂质和天然或经胰蛋白酶处理的细胞色素b5组成的囊泡的H2O-D2O混合物中收集小角中子衍射图案。从这些数据中得出囊泡中散射密度径向分布的二阶矩，并与从三个提出的模型计算出的值进行比较，这三个模型因细胞色素b5穿透脂质双层的程度不同而有所差异。蛋白质疏水结构域分布在双层中的模型与数据最为吻合。
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