Van Dijk A M, Laird J D
Blood Vessels. 1984;21(6):267-78. doi: 10.1159/000158529.
Single smooth muscle cells from bovine coronary artery were obtained by enzymatic digestion. 20.1 +/- 3.2% (mean value of seven experiments) of the dispersed cells were in a relaxed, noncontracted state. These cells responded to electrically induced depolarization with a graded contraction to a maximum shortening of 47.8% of initial length; the corresponding maximum for K+-induced depolarization was 61.7% of initial length. Cells relaxed only a small percentage (approximately equal to 20%) of the amount of shortening, but could be restimulated several times to contract to even shorter lengths. The decrease in mean cell length of dispersed cells exposed to serotonin, acetylcholine, and ergonovine maleate was 21.0, 23.0 and 25.5%, respectively (p less than 0.05). No significant decrease in cell length was obtained with norepinephrine. The ultrastructure of the relaxed cells seemed to be unaltered, suggesting that the accuracy of the physiological results is not impaired by induced structural damage.
通过酶消化获得牛冠状动脉的单个平滑肌细胞。20.1±3.2%（七次实验的平均值）的分散细胞处于松弛、未收缩状态。这些细胞对电诱导的去极化产生分级收缩，最大缩短至初始长度的47.8%；钾离子诱导去极化的相应最大值为初始长度的61.7%。细胞仅松弛缩短量的一小部分（约等于20%），但可被多次重新刺激以收缩至更短长度。暴露于血清素、乙酰胆碱和马来酸麦角新碱的分散细胞平均细胞长度分别减少21.0%、23.0%和25.5%（p<0.05）。去甲肾上腺素未使细胞长度出现显著减少。松弛细胞的超微结构似乎未改变，这表明诱导的结构损伤未损害生理结果的准确性。
