Iskandar D, Bonhomme F
Can J Genet Cytol. 1984 Oct;26(5):622-7. doi: 10.1139/g84-096.
Forty-six rodent species of the Muridae family were submitted to sequential electrophoresis for the study of 11 protein loci, using 12 buffer systems which differed in pH and ionic composition. The complete set of electrophoretic conditions yielded 135 variants of which 68 were detected through a single condition. The twofold increase of revealed variants was essentially limited to intergeneric comparisons because few additional variants within genera were revealed, despite the use of several buffers. These results show that estimation of the degree of genetic differentiation among taxa at a higher level than that of the genus by standard electrophoretic data may, although of current use, lead to erroneous results.
对鼠科的46种啮齿动物进行了连续电泳,以研究11个蛋白质位点,使用了12种pH值和离子组成不同的缓冲系统。整套电泳条件产生了135个变体,其中68个是通过单一条件检测到的。尽管使用了几种缓冲液,但揭示的变体数量增加两倍主要局限于属间比较,因为属内几乎没有发现更多的变体。这些结果表明,通过标准电泳数据估计高于属水平的分类单元之间的遗传分化程度,虽然目前仍在使用,但可能会导致错误的结果。