Hollander D, Chow S L, Dadufalza V D
Can J Physiol Pharmacol. 1984 Sep;62(9):1136-40. doi: 10.1139/y84-190.
The intestinal absorption of free oleic acid at low intraluminal concentrations and the influence of luminal factors on its absorption were studied in the unanesthetized rat. The relationship between oleic acid concentration (30-2500 microM) and its rate of absorptions fitted best to a rectangular hyperbola (y = x/(2.19 + 0.0015x), r = 0.94). Oleic acid's rate of absorption increased as the hydrogen ion and sodium taurocholate concentrations were increased or as the thickness and resistance of the unstirred water layer were diminished or following the addition of lysolecithin. The additions of the artificial detergent Tween-80, or lecithin and linoleic, linolenic and arachidonic acids to the perfusate decreased oleic acid's rate of absorption. It was concluded that oleic acid absorption in this range of concentrations displays apparent saturation kinetics which are due to unstirred layer effects, limited aqueous solubility of oleic acid and possible saturation of cytosol fatty acid binding proteins. Factors which increase oleic acid's protonated concentration or diminish the unstirred layer resistance, enhance its absorption rate, while factors which enhance its micellar solubility or interfere with its transfer out of the cell membrane decrease its overall rate of absorption.
在未麻醉的大鼠中研究了低腔内浓度下游离油酸的肠道吸收及其吸收过程中腔内因素的影响。油酸浓度（30 - 2500微摩尔）与其吸收速率之间的关系最符合矩形双曲线（y = x/(2.19 + 0.0015x)，r = 0.94）。随着氢离子和牛磺胆酸钠浓度的增加、未搅动水层的厚度和阻力减小或添加溶血卵磷脂后，油酸的吸收速率增加。向灌注液中添加人工洗涤剂吐温80、卵磷脂以及亚油酸、亚麻酸和花生四烯酸会降低油酸的吸收速率。得出的结论是，在此浓度范围内油酸的吸收表现出明显的饱和动力学，这是由于未搅动层效应、油酸有限的水溶性以及细胞质脂肪酸结合蛋白可能的饱和所致。增加油酸质子化浓度或降低未搅动层阻力的因素会提高其吸收速率，而增加其胶束溶解度或干扰其从细胞膜转运出的因素会降低其总体吸收速率。
