Trnka P L, Pergament E, Anderson N G
Clin Chem. 1984 Dec;30(12 Pt 1):2040-2.
Two-dimensional electrophoresis was applied to specimens of human chorionic villi obtained during the first trimester of gestation, the object being to simultaneously map several hundred polypeptide gene products. Genetically normal specimens were homogenized in a urea-based denaturant and the supernates were electrophoresed with use of the "ISO-DALT" system. Four categories of proteins are distinguished on the map: previously identified proteins present in chorionic villi and other cell types; unidentified proteins present in chorionic villi and other cell types; proteins present in chorionic villi and amniotic fluid but not in other cell types; and proteins probably originating from the amnio-chorionic plate. The reference map for chorionic villi provided in this study may serve as the basis of determining whether genetic analyses conducted in the first trimester accurately represent the fetal genotype.
二维电泳应用于妊娠早期获取的人绒毛膜绒毛标本，目的是同时绘制数百种多肽基因产物图谱。将基因正常的标本在基于尿素的变性剂中匀浆，然后使用“ISO-DALT”系统对上清液进行电泳。在图谱上区分出四类蛋白质：绒毛膜绒毛和其他细胞类型中存在的先前已鉴定的蛋白质；绒毛膜绒毛和其他细胞类型中存在的未鉴定蛋白质；绒毛膜绒毛和羊水但其他细胞类型中不存在的蛋白质；以及可能起源于羊膜绒毛板的蛋白质。本研究提供的绒毛膜绒毛参考图谱可作为确定孕早期进行的基因分析是否准确代表胎儿基因型的基础。
