Jacobson B
Exp Eye Res. 1984 Sep;39(3):373-85. doi: 10.1016/0014-4835(84)90025-3.
The ability of extracts of calf vitreous hyalocytes to catalyze the degradation of glycosaminoglycans was studied by incubation with radioactively labeled substrates. The degradation of the polymeric substrates to lower molecular weight products was assayed by three methods: (1) paper chromatographic separation of low molecular weight, mobile digestion products from undigested, high molecular weight material which remains at or near the origin; (2) loss of the ability of the glycosaminoglycan to be precipitated by cetylpyridinium chloride; (3) gel chromatography to separate low molecular weight digestion products, which appear in the retarded volume, from undegraded, high molecular weight material, which is eluted in the void volume. The acidic pH optimum of the reaction suggests a lysosomal origin of the enzyme activity.
通过与放射性标记的底物一起孵育，研究了小牛玻璃体透明细胞提取物催化糖胺聚糖降解的能力。通过三种方法测定聚合物底物降解为低分子量产物的情况：（1）纸色谱法分离低分子量、可移动的消化产物与未消化的、留在原点或原点附近的高分子量物质；（2）糖胺聚糖被十六烷基吡啶氯化物沉淀的能力丧失；（3）凝胶色谱法分离出现在延迟体积中的低分子量消化产物与在空体积中洗脱的未降解高分子量物质。反应的最适酸性pH表明酶活性起源于溶酶体。
