Rikihisa Y, Letonja T, Pratt N, Chin Lin Y
Exp Parasitol. 1984 Dec;58(3):230-8. doi: 10.1016/0014-4894(84)90039-0.
Development of larvae of the cestode parasite Taenia taeniaeformis in the liver of rats induces gross hyperplasia of the gastric mucosa and excessive mucus production in the stomach without any direct contact with the stomach. Because the taeniid larvae are known to elaborate excretory-secretory (E-S) product in vivo and in vitro, the product was analyzed further, and its effects on cultured rat and dog stomach cells were investigated. In vitro E-S product contained less negatively charged glycosaminoglycan than either heparin or chondroitin sulfate, and proteins of various molecular weights. It stimulated the growth of both rat and dog stomach cells at concentrations of 3-9 micrograms protein/ml culture medium. At a concentration of 30 micrograms protein/ml culture medium, it stimulated hexosamine production in the cells up to 20 times, and multiple intracytoplasmic granules were found in both rat and dog cultured cells by light and electron microscopy. These results suggest that larval E-S product may be involved in the induction of gastric hyperplasia and hypermucus secretion.
绦虫寄生虫——泡状带绦虫的幼虫在大鼠肝脏内发育，可诱导胃黏膜出现明显增生，且在未与胃直接接触的情况下，使胃内产生过多黏液。由于已知带科幼虫可在体内和体外产生排泄-分泌（E-S）产物，因此对该产物进行了进一步分析，并研究了其对培养的大鼠和犬胃细胞的影响。体外E-S产物所含带负电荷的糖胺聚糖比肝素或硫酸软骨素少，且含有各种分子量的蛋白质。在培养基中蛋白质浓度为3-9微克/毫升时，它可刺激大鼠和犬胃细胞的生长。在培养基中蛋白质浓度为30微克/毫升时，它可使细胞中的己糖胺生成量增加多达20倍，通过光学显微镜和电子显微镜观察发现，在培养的大鼠和犬细胞中均有多个胞浆内颗粒。这些结果表明，幼虫的E-S产物可能与胃增生和黏液分泌过多的诱导有关。
