Benchimol M, de Souza W
Exp Parasitol. 1984 Dec;58(3):356-64. doi: 10.1016/0014-4894(84)90052-3.
The polyene antibiotic, filipin, was used as a probe for the detection of sterols in the freeze-fractured plasma membrane and the flagellar membranes of the pathogenic protozoa, Tritrichomonas foetus. A homogeneous distribution of filipin-sterol complexes was seen throughout the plasma membrane, and the membrane of the three anterior and the one recurrent flagella. No or very few filipin-sterol complexes were observed in some specialized regions such as the base of the flagella (necklace), the portion of the recurrent flagellum, and that part of the cell body to which the flagellum was attached. The density of filipin-sterol complexes varied from one cell to the other. In some cells, about 205 complexes/micron 2 were seen. A larger number of filipin-sterol complexes were observed on both faces of the membrane of cytoplasmic structures, probably corresponding to vacuoles. No complexes were seen in the nuclear membrane and in the membrane of the endoplasmic reticulum. Very few or no complexes were observed in the membrane of the hydrogenosomes. Treatment of living cells with filipin induced aggregation of filipin-sterol complexes at some points of the plasma membrane.
多烯抗生素制霉菌素被用作探针，用于检测致病性原生动物胎儿三毛滴虫冷冻断裂的质膜和鞭毛膜中的固醇。在整个质膜以及三根前鞭毛和一根后鞭毛的膜中都观察到制霉菌素 - 固醇复合物的均匀分布。在一些特殊区域，如鞭毛基部（项链状结构）、后鞭毛部分以及鞭毛附着的细胞体部分，未观察到或仅观察到极少的制霉菌素 - 固醇复合物。制霉菌素 - 固醇复合物的密度在不同细胞之间有所变化。在一些细胞中，每平方微米可见约205个复合物。在细胞质结构膜的两面观察到大量的制霉菌素 - 固醇复合物，可能对应于液泡。在核膜和内质网膜中未观察到复合物。在氢化酶体膜中观察到极少或没有复合物。用制霉菌素处理活细胞会导致制霉菌素 - 固醇复合物在质膜的某些点聚集。
