Gershwin M E, Lentz D, Owens R B
Exp Cell Biol. 1984;52(6):361-70. doi: 10.1159/000163283.
There are many factors, other than the absence of an immune response, that are responsible for the successful transplantation of xenografts to nude mice. We have defined, in this study, the relationship between karyotype and transplantability of xenografts into nude mice, using a library of 85 tumor and normal tissue culture lines. Each of these lines had been previously passaged in tissue culture for more than 10 generations. 53 of these 85 lines were classified as diploid, whereas 32 lines were classified as heteroploid. These tissue culture lines were transplanted to groups of 4-10 unmanipulated and antilymphocyte serum (ALS)-treated nude mice and serially monitored for 12 weeks. Only 19% of the diploid lines were successfully transplanted into unmanipulated nude mice, and only 30% were successfully transplanted into ALS-treated nude mice. In comparison, 50% of the heteroploid lines were successfully grafted into unmanipulated nude mice, and a total of 60% were successfully transplanted into ALS-treated nude ice. 12 of the 53 diploid lines can be classified as being pseudodiploid on the basis of rearranged chromosomes or other minor karyotype anomalies. 8 of these 12 pseudodiploid lines were successfully transplanted into nude mice, further strengthening the relationship between abnormal karyotype and transplantability. Tumor karyotype is an additional parameter which should be added to the variables involved in the successful transplantation of tumors to nude mice.
除了缺乏免疫反应外，还有许多因素导致异种移植物能够成功移植到裸鼠体内。在本研究中，我们利用包含85种肿瘤和正常组织培养系的文库，确定了异种移植物的核型与移植到裸鼠体内的可移植性之间的关系。这些系中的每一个此前都已在组织培养中传代超过10代。这85个系中有53个被分类为二倍体，而32个系被分类为异倍体。将这些组织培养系移植到4至10只未处理和经抗淋巴细胞血清（ALS）处理的裸鼠组中，并连续监测12周。只有19%的二倍体系能够成功移植到未处理的裸鼠体内，只有30%能够成功移植到经ALS处理的裸鼠体内。相比之下，50%的异倍体系能够成功移植到未处理的裸鼠体内，总共60%能够成功移植到经ALS处理的裸鼠体内。53个二倍体系中有12个基于染色体重排或其他轻微核型异常可被分类为假二倍体。这12个假二倍体系中有8个成功移植到了裸鼠体内，进一步强化了异常核型与可移植性之间的关系。肿瘤核型是一个应添加到肿瘤成功移植到裸鼠所涉及变量中的额外参数。
