大鼠唾液腺酸盐水提取物中125I-胰高血糖素降解活性

125I-glucagon-degrading activity in acid-saline extracts of rat salivary gland.

作者信息

Tominaga M, Yamatani K, Marubashi S, Kaneda H, Manaka H, Kamimura T, Katagiri T, Sasaki H

出版信息

Diabetologia. 1984 Sep;27(3):392-6. doi: 10.1007/BF00304856.

Abstract

The antibody-binding ability of the glucagon-like substance in rat submaxillary gland acid saline extract was examined by affinity chromatography, and the biological activity studied using the isolated liver perfusion method. We found that the glucagon-like substances in acid saline extract could not be bound to anti-glucagon antibody and that the gel-filtration peak on ultrogel AcA 54 could increase neither glucose nor cyclic AMP output from isolated perfused rat liver. Furthermore, the radioactivity peak of 125I-glucagon on Bio Gel P-6 column chromatography moved from its original position and eluted in later fractions after incubation with an acid saline extract of the submaxillary gland. In consequence, there was 125I-glucagon degrading activity in the submaxillary gland, but no glucagon-related peptide. Therefore, it is suggested that the glucagon-like substance, which has been reported in acid saline extract of the rat salivary gland, may be an artifact due to tracer degrading activity.

摘要

通过亲和色谱法检测大鼠颌下腺酸性盐水提取物中胰高血糖素样物质的抗体结合能力，并使用离体肝脏灌注法研究其生物活性。我们发现酸性盐水提取物中的胰高血糖素样物质不能与抗胰高血糖素抗体结合，并且在超凝胶AcA 54上的凝胶过滤峰既不能增加离体灌注大鼠肝脏的葡萄糖输出，也不能增加环磷酸腺苷输出。此外，在与颌下腺酸性盐水提取物孵育后，Bio Gel P - 6柱色谱上125I - 胰高血糖素的放射性峰从其原始位置移动并在较晚的馏分中洗脱。因此，颌下腺中存在125I - 胰高血糖素降解活性，但不存在胰高血糖素相关肽。所以，有人提出在大鼠唾液腺酸性盐水提取物中报道的胰高血糖素样物质可能是由于示踪剂降解活性导致的假象。