Divoll M, Greenblatt D J, Arendt R M
Int J Clin Pharmacol Ther Toxicol. 1984 Sep;22(9):457-60.
Concentrations of propranolol in plasma or cerebrospinal fluid can be reliably quantitated using liquid chromatography with fluorescence detection. After addition of pronethalol as internal standard, alkalinized samples are extracted with hexane:isoamyl alcohol (98:2); the organic extract is separated, evaporated to dryness, reconstituted in mobile phase (acetonitrile:water:acetic acid, 30:69:1), and chromatographed on a reverse-phase C-18 column. Effluent is monitored with fluorescence detection at excitation and emission wavelengths of 235 and 335 nm. The simplified extraction procedure plus an automatic injection system allows one person to analyze up to 100 samples per day. Sensitivity limits are .5 ng of propranolol per ml of sample, and the method is applicable to experimental and clinical pharmacokinetic studies.
使用带荧光检测的液相色谱法可以可靠地定量血浆或脑脊液中普萘洛尔的浓度。加入普萘洛尔作为内标后，用己烷：异戊醇（98:2）对碱化的样品进行萃取；分离有机萃取物，蒸发至干，用流动相（乙腈：水：乙酸，30:69:1）复溶，并在反相C-18柱上进行色谱分析。在激发波长和发射波长分别为235和335 nm的条件下，用荧光检测监测流出物。简化的萃取程序加上自动进样系统使一人每天最多可分析100个样品。灵敏度极限为每毫升样品0.5纳克普萘洛尔，该方法适用于实验和临床药代动力学研究。
