Biesalski H K
Int J Vitam Nutr Res. 1984;54(2-3):113-8.
Chemical separation of different vitamin A derivates in inner ear extracts was performed by means of high pressure liquid chromatography (HPLC) with fluorescence and UV detection. For more accurate site identification the membranous cochlea was dissected and the basilar membrane, the spiral ligament and the stria vascularis were isolated for analysis. Retinyl esters were found in the basilar membrane at a concentration of 11.4 +/- 5.9 micrograms/g dry weight, a high concentration compared with that in other tissues of other organs. The spiral ligament contained retinyl esters at a lesser concentration and no retinyl esters were found in the stria vascularis. Retinol was not detected in the spiral ligament or basilar membrane but was found in the stria vascularis at a concentration of 1.2 +/- 0.8 micrograms/g. Retinyl palmitate and retinyl stearate were identified. This investigation shows that vitamin A forms retinyl esters with long-chain fatty acids in the basilar membrane in high concentrations relative to other organs and in greater quantity than in other sites in the inner ear.
采用带荧光和紫外检测的高压液相色谱法（HPLC）对内耳提取物中的不同维生素A衍生物进行化学分离。为了更准确地确定位置，解剖了膜性耳蜗，并分离出基底膜、螺旋韧带和血管纹进行分析。在基底膜中发现视黄酯，浓度为11.4±5.9微克/克干重，与其他器官的其他组织相比，该浓度较高。螺旋韧带中视黄酯的浓度较低，而在血管纹中未发现视黄酯。在螺旋韧带或基底膜中未检测到视黄醇，但在血管纹中发现其浓度为1.2±0.8微克/克。鉴定出了棕榈酸视黄酯和硬脂酸视黄酯。这项研究表明，相对于其他器官，维生素A在基底膜中与长链脂肪酸形成视黄酯的浓度较高，且比内耳的其他部位含量更多。
