液相色谱法测定猪肉组织中的卡巴氧、脱氧卡巴氧和呋喃西林类药物。

Liquid chromatographic determination of carbadox, desoxycarbadox, and nitrofurazones in pork tissues.

作者信息

MacIntosh A I, Neville G A

出版信息

J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):958-62.

Abstract

A liquid chromatographic (LC) method has been developed for the determination of carbadox, desoxycarbadox, and nitrofurazones in the 10-40 ppb range in pork muscle, liver, and kidney tissues. Tissues were homogenized in absolute ethanol, and the homogenates were treated with metaphosphoric acid and reduced in volume by rotovaporization. Hexane was added to the concentrates, which were then centrifuged to remove fat. After addition of KH2PO4 to the aqueous phase and extraction with ethyl acetate, the extracts were passed through alumina columns before analysis by reverse phase LC. Overall average recoveries (10-40 ppb range) for carbadox and desoxycarbadox from spiked tissues were 53% +/- 13.6 and 61% +/- 7.2, respectively; overall average recoveries for nitrofurazone and furazolidone were 43% +/- 7.3 and 77% +/- 10.9, respectively. Before these optimum determinations, degradation by even minimal incandescent light was found to reduce recovery especially of desoxycarbadox. The results of this photochemical degradation are reported and briefly discussed.

摘要

已开发出一种液相色谱（LC）方法，用于测定猪肌肉、肝脏和肾脏组织中浓度范围在10 - 40 ppb的卡巴多司、脱氧卡巴多司和硝基呋喃类药物。将组织在无水乙醇中匀浆，匀浆物用偏磷酸处理并通过旋转蒸发浓缩。向浓缩物中加入己烷，然后离心以去除脂肪。向水相中加入KH2PO4并用乙酸乙酯萃取后，萃取物在通过氧化铝柱后再进行反相LC分析。加标组织中卡巴多司和脱氧卡巴多司的总体平均回收率（10 - 40 ppb范围）分别为53%±13.6和61%±7.2；硝基呋喃和呋喃唑酮的总体平均回收率分别为43%±7.3和77%±10.9。在进行这些最佳测定之前，发现即使是极少量的白炽光降解也会降低回收率，尤其是脱氧卡巴多司的回收率。本文报道并简要讨论了这种光化学降解的结果。