透明质酸寡糖的薄层色谱法。
Thin-layer chromatography of hyaluronate oligosaccharides.
作者信息
Shimada E, Matsumura G
出版信息
J Biochem. 1984 Sep;96(3):721-5. doi: 10.1093/oxfordjournals.jbchem.a134890.
Abstract
Odd- and even-numbered hyaluronate oligosaccharides with N-acetylglucosamine, glucuronic acid, or 4,5-unsaturated glucuronic acid at their nonreducing ends were separated by thin-layer chromatography, using silica gel and a solvent system of isopropanol-water (66 : 34) containing 0.05 M NaCl. In the isopropanol system, small amounts of electrolytes were necessary for the resolution of each oligosaccharide.
摘要
通过薄层色谱法，使用硅胶以及含有0.05M氯化钠的异丙醇 - 水（66:34）溶剂系统，分离在其非还原端带有N - 乙酰葡糖胺、葡糖醛酸或4,5 - 不饱和葡糖醛酸的奇数和偶数低聚透明质酸。在异丙醇系统中，少量电解质对于每种低聚糖的分离是必要的。
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