Bremer E G, Levery S B, Sonnino S, Ghidoni R, Canevari S, Kannagi R, Hakomori S
J Biol Chem. 1984 Dec 10;259(23):14773-7.
The antigen defined by a monoclonal antibody, MBr1, was found to be expressed in normal human mammary gland epithelia and human mammary carcinoma cells (Ménard, S., Tagliabue, E., Canevari, S., Fossati, G., and Colnaghi, M. I. (1983) Cancer Res. 43, 1295-1300). The antigen has been isolated from breast cancer cell line MCF-7, which was used as immunogen, and its structure was determined by methylation analysis, NMR spectroscopy, direct probe mass spectrometry, and enzymatic degradation as identified below. Fuc alpha 1----2Gal beta 1----3GalNAc beta 1----3Gal alpha 1----4Gal beta 1----4Glc beta 1----1Cer The antibody cross-reacted weakly with fucosylasialo-GM1 (IV2FucGg4), which shares the same terminal sequence, Fuc alpha 1----2Gal beta 1----3GalNAc, with this antigen. However, various other structures, including lacto-series H structure (Fuc alpha 1----2 Gal beta 1----4/or 3GlcNAc beta 1----3Gal), did not show any reactivity with this antibody. Therefore, this antigen represents a blood group H antigen with a globo-series structure which is abundant in human teratocarcinoma (Kannagi, R., Levery, S. B., Ishigami, F., Hakomori, S., Shevinsky, L. H., Knowles, B. B., and Solter, D. (1983) J. Biol. Chem. 258, 8934-8942), although its presence must be limited in normal adult human tissue.
一种由单克隆抗体MBr1所定义的抗原，被发现表达于正常人乳腺上皮细胞和人乳腺癌细胞中（梅纳尔，S.，塔利亚布埃，E.，卡内瓦里，S.，福萨蒂，G.，以及科尔纳吉，M.I.（1983年）《癌症研究》43卷，1295 - 1300页）。该抗原已从乳腺癌细胞系MCF - 7中分离出来，MCF - 7被用作免疫原，其结构通过甲基化分析、核磁共振光谱、直接探针质谱分析以及如下所述的酶促降解来确定。岩藻糖α1----2半乳糖β1----3N - 乙酰半乳糖胺β1----3半乳糖α1----4半乳糖β1----4葡萄糖β1----1神经酰胺 该抗体与岩藻糖基唾液酸 - GM1（IV2FucGg4）有弱交叉反应，岩藻糖基唾液酸 - GM1与该抗原具有相同的末端序列，即岩藻糖α1----2半乳糖β1----3N - 乙酰半乳糖胺。然而，包括乳糖系列H结构（岩藻糖α1----2半乳糖β1----4/或3N - 乙酰葡糖胺β1----3半乳糖）在内的各种其他结构，与该抗体均无任何反应性。因此，这种抗原代表一种具有球系列结构的血型H抗原，在人畸胎瘤中含量丰富（金波，R.，勒弗里，S.B.，石上，F.，滨木本，S.，谢文斯基，L.H.，诺尔斯，B.B.，以及索尔特，D.（1983年）《生物化学杂志》258卷，8934 - 8942页），尽管其在正常成人组织中的存在必定有限。
