Korant B D, Blomstrom D C, Jonak G J, Knight E
J Biol Chem. 1984 Dec 10;259(23):14835-9.
Human interferons induce a protein of 15,000 daltons in human and bovine cells. This protein is located in the cytoplasm in a soluble form and is induced by concentrations of interferon which induce the antiviral state. Messenger RNA prepared from interferon-treated human and bovine cells contains a mRNA which yields on translation in vitro a protein similar in size to the 15-kDa protein induced by interferon in vivo. The human protein has been purified to homogeneity from interferon-treated human cells by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. A comparison of the peptides generated by V8 protease from the human and bovine 15-kDa proteins reveals that the two proteins are similar but not identical.
人干扰素可在人细胞和牛细胞中诱导产生一种15000道尔顿的蛋白质。这种蛋白质以可溶形式存在于细胞质中，并且在诱导抗病毒状态的干扰素浓度下被诱导产生。从经干扰素处理的人细胞和牛细胞中制备的信使核糖核酸（mRNA）含有一种mRNA，该mRNA在体外翻译时产生一种大小与干扰素在体内诱导产生的15 kDa蛋白质相似的蛋白质。通过离子交换色谱法和反相高效液相色谱法已从经干扰素处理的人细胞中纯化出了人源蛋白质，使其达到了均一性。对V8蛋白酶作用于人源和牛源15 kDa蛋白质所产生的肽段进行比较后发现，这两种蛋白质相似但并不相同。
