Maître J M, Boss G, Testa B
J Chromatogr. 1984 Sep 21;299(2):397-403. doi: 10.1016/s0021-9673(01)97855-0.
The enantiomers of 2-phenylpropionic acid, 2-(2-naphthyl)propionic acid, 2-(4-biphenyl)propionic acid and six anti-inflammatory congeners were separated by high-performance liquid chromatography via their diastereoisomeric derivatives with (S)-(-)-1-phenylethylamine. In agreement with a general rule, the diastereoisomers derived from the (R)-acids are less polar and elute first. Structural factors influencing the resolution are discussed. Good calibrations were obtained for R/S ratios and total (R + S) concentrations of flurbiprofen and naproxen added to inactivated rat liver preparations. The method is suitable for in vitro metabolic studies of chiral 2-arylpropionates.
通过与(S)-(-)-1-苯乙胺形成非对映体衍生物，利用高效液相色谱法分离了2-苯丙酸、2-(2-萘基)丙酸、2-(4-联苯基)丙酸的对映体以及六种抗炎同系物。与一般规律一致，由(R)-酸衍生的非对映体极性较小，先被洗脱。讨论了影响拆分的结构因素。对于添加到灭活大鼠肝脏制剂中的氟比洛芬和萘普生的R/S比值和总(R+S)浓度，获得了良好的校准曲线。该方法适用于手性2-芳基丙酸酯的体外代谢研究。
