Driscoll J N, Conron D W, Ferioli P, Krull I S, Xie K H
J Chromatogr. 1984 Oct 19;302:43-50. doi: 10.1016/s0021-9673(01)88997-4.
Photoionization detection (PID) has now been successfully interfaced with modern high-performance liquid chromatography (HPLC) to provide a new "hyphenated" technique, HPLC-PID. This method is compatible with reversed-phase HPLC solvents and conditions, as well as with certain organic solvents used in normal-phase liquid chromatography. The HPLC-PID interface consists of a variable-ratio eluent splitter, operated inside a heated oven, which vaporizes both analytes and mobile phase prior to introduction into the detector. Depending on the energy (eV) of the photoionization detector lamp, most of the solvents commonly used in reversed-phase liquid chromatography are compatible with long-term, on-line, real-time, continuous photoionization detector operation. Virtually all organic classes now detectable by gas chromatography-PID can also be detected by HPLC-PID, but with somewhat poorer detection limits. In certain instances, minimum detection limits are in the 5-500 ng/injection range. The HPLC-PID system appears ideal for aromatic or aliphatic amines, substituted hydrocarbons, and certain other classes of organic compounds. Various applications of HPLC-PID, under reversed-phase conditions are illustrated and discussed.
光离子化检测（PID）现已成功与现代高效液相色谱（HPLC）联用，形成了一种新的“联用”技术——HPLC - PID。该方法与反相HPLC溶剂和条件兼容，也与正相液相色谱中使用的某些有机溶剂兼容。HPLC - PID接口由一个可变比例洗脱液分流器组成，在加热炉内运行，它在将分析物和流动相引入检测器之前将它们汽化。根据光离子化检测器灯的能量（eV），反相液相色谱中常用的大多数溶剂都与光离子化检测器的长期在线实时连续运行兼容。气相色谱 - PID目前能检测的几乎所有有机类别，HPLC - PID也都能检测，但检测限稍差。在某些情况下，最低检测限在5 - 500 ng/进样范围内。HPLC - PID系统似乎非常适合用于检测芳香族或脂肪族胺、取代烃以及某些其他类别的有机化合物。文中展示并讨论了反相条件下HPLC - PID的各种应用。
