Weissberger L E, Armstrong M K
J Chromatogr Sci. 1984 Oct;22(10):438-40. doi: 10.1093/chromsci/22.10.438.
The purpose of this project was to isolate and quantitate canavanine in three different alfalfa samples because it has been hypothesized that alfalfa and therefore canavanine ingestion may exacerbate systemic lupus erythematosus (SLE) in humans. Ten alfalfa tablets were ground into a fine powder, and 100 mg was extracted for canavanine. The extracted canavanine was derivatized prior to quantitation by high performance liquid chromatography. The canavanine content of alfalfa tablets averaged 25.3 and 33.8 micrograms/g depending on lot number and extraction method. Because ten alfalfa tablets are recommended daily, this would provide approximately 0.18 mg of canavanine. Whether daily ingestion of canavanine exacerbated SLE in two patients consuming 15 and 8 alfalfa tablets (0.27 and 0.15 mg, respectively) is uncertain and warrants further investigation.
本项目的目的是分离并定量三种不同苜蓿样品中的刀豆氨酸，因为据推测，苜蓿以及因此摄入的刀豆氨酸可能会加重人类的系统性红斑狼疮（SLE）。将十片苜蓿片剂研磨成细粉，取100毫克用于提取刀豆氨酸。在通过高效液相色谱法定量之前，对提取的刀豆氨酸进行衍生化处理。根据批次号和提取方法，苜蓿片剂中刀豆氨酸的含量平均为25.3和33.8微克/克。由于建议每日服用十片苜蓿片剂，这将提供约0.18毫克的刀豆氨酸。每日摄入刀豆氨酸是否会加重两名分别服用15片和8片苜蓿片剂（分别为0.27毫克和0.15毫克）的患者的系统性红斑狼疮尚不确定，值得进一步研究。
