Soares R M, Alviano C S, Silva-Filho F C, Esteves M J, Angluster J, De Souza W
J Submicrosc Cytol. 1984 Oct;16(4):735-9.
The influence of dimethylsulfoxide (DMSO) on surface components (Con A receptors, antigenic sites recognized by monospecific antibodies and membrane associated polysaccharide components) of Herpetomonas samuelpessoai was investigated. Techniques such as light and scanning electron microscopy and gas-liquid chromatography were used. The characteristic flagellar-flagellar (F-F) pattern of cell agglutination with Con A was markedly modified by DMSO and besides the F-F interactions other types of agglutination, including flagellar-somatic (F-S) and somatic-somatic (S-S), were detected. DMSO induced significant antigenic alteration in H. samuelpessoai. The interaction with serum anti-flagellate was greatly decreased in DMSO-treated cells. The pattern of the composition of membrane-associated polysaccharides was also substantially changed: DMSO induced a preferential synthesis of galactose and a decrease of xylose, whereas mannose and glucose content remained practically unchanged.
研究了二甲基亚砜（DMSO）对塞缪尔佩索阿赫氏鞭毛虫表面成分（伴刀豆球蛋白A受体、单特异性抗体识别的抗原位点和膜相关多糖成分）的影响。使用了光学显微镜、扫描电子显微镜和气液色谱等技术。DMSO显著改变了伴刀豆球蛋白A诱导的细胞凝集特征性鞭毛-鞭毛（F-F）模式，除了F-F相互作用外，还检测到其他类型的凝集，包括鞭毛-体细胞（F-S）和体细胞-体细胞（S-S）。DMSO诱导了塞缪尔佩索阿赫氏鞭毛虫显著的抗原改变。在经DMSO处理的细胞中，与抗鞭毛虫血清的相互作用大大降低。膜相关多糖的组成模式也发生了显著变化：DMSO诱导半乳糖优先合成，木糖减少，而甘露糖和葡萄糖含量基本保持不变。
