Ito-Kuwa S, Aoki S
Microbiol Immunol. 1984;28(8):893-901. doi: 10.1111/j.1348-0421.1984.tb00745.x.
The effects of fermenting, poorly arginine-utilizing Mycoplasma fermentans and arginine-utilizing Mycoplasma salivarium on the frequency of sister chromatid exchange (SCE) in cultured human lymphocytes were examined. M. fermentans caused no apparent mitosis inhibition of lymphocytes and the increase in SCE frequency was dependent on the inoculum size of the mycoplasma. An evident increase in SCE frequency was observed in lymphocytes infected with smaller inoculum sizes of M. salivarium whereas there was mitosis inhibition of lymphocytes infected with larger inoculum sizes of the mycoplasma. In lymphocyte cultures infected with M. salivarium, the addition of arginine to the culture medium reduced mitosis inhibition but did not diminish the increase in SCE frequency, indicating that arginine depletion was not involved in causing the induction of SCEs in mycoplasma-infected lymphocytes. With regard to the genetic effectiveness of SCE, these results suggested that mycoplasmas are capable of inducing cytogenetic changes in infected host cells.
研究了发酵型、精氨酸利用能力差的发酵支原体以及利用精氨酸的唾液支原体对培养的人淋巴细胞中姐妹染色单体交换（SCE）频率的影响。发酵支原体对淋巴细胞无明显的有丝分裂抑制作用，SCE频率的增加取决于支原体的接种量。在接种较小量唾液支原体的淋巴细胞中观察到SCE频率明显增加，而接种较大量该支原体的淋巴细胞则出现有丝分裂抑制。在感染唾液支原体的淋巴细胞培养物中，向培养基中添加精氨酸可减轻有丝分裂抑制，但并未减少SCE频率的增加，这表明精氨酸耗竭与支原体感染的淋巴细胞中SCE的诱导无关。关于SCE的遗传效应，这些结果表明支原体能够在受感染的宿主细胞中诱导细胞遗传学变化。
