Drebin J A, Stern D F, Link V C, Weinberg R A, Greene M I
Nature. 1984;312(5994):545-8. doi: 10.1038/312545a0.
A variety of antigens have been identified on the surface of the malignant cell. However, identical antigens are often found on non-malignant cells of the same or different histological origin, or of a different stage of embryonic development. Many of these tumour-associated antigens appear to be only incidentally expressed on neoplastic cells. Clearly, it would be of great interest to identify cell-surface antigens whose expression is associated specifically with the transformed state and linked directly with the mechanisms responsible for transformation. The detection of activated cellular oncogenes in human and animal cancer cells by the technique of DNA transfection has allowed the isolation of genetic elements which are thought to have a critical role in malignancy. Here, in an effort to identify cell-surface antigens associated with the neoplastic process, we have generated hybridomas which secrete monoclonal antibodies that react specifically with cell-surface determinants found on NIH 3T3 cells transformed by transfection with a group of rat neuroblastoma oncogenes. These antibodies bind to and immunoprecipitate a phosphoprotein of relative molecular mass 185,000 (185 K) from a DNA donor rat neuroblastoma and 13 independent rat neuroblastoma DNA transfectants. There was no antibody reactivity with normal NIH 3T3 cells or with NIH 3T3 cells transformed by various other agents.
已在恶性细胞表面鉴定出多种抗原。然而，在相同或不同组织学来源、或处于胚胎发育不同阶段的非恶性细胞上，常常能发现相同的抗原。许多这类肿瘤相关抗原似乎只是偶然在肿瘤细胞上表达。显然，鉴定那些其表达与转化状态特异性相关且直接与转化机制相联系的细胞表面抗原，将会非常有意义。通过DNA转染技术在人和动物癌细胞中检测活化的细胞癌基因，已使得人们能够分离出被认为在恶性肿瘤中起关键作用的遗传元件。在此，为了鉴定与肿瘤形成过程相关的细胞表面抗原，我们制备了杂交瘤，这些杂交瘤分泌的单克隆抗体能与用一组大鼠神经母细胞瘤癌基因转染而转化的NIH 3T3细胞上发现的细胞表面决定簇发生特异性反应。这些抗体能结合并免疫沉淀来自DNA供体大鼠神经母细胞瘤和13个独立的大鼠神经母细胞瘤DNA转染细胞的相对分子质量为185,000（185K）的一种磷蛋白。正常NIH 3T3细胞或由其他各种因子转化的NIH 3T3细胞均无抗体反应性。
