Benzi G, Villa R F, Dossena M, Vercesi L, Gorini A, Pastoris O
Neurochem Res. 1984 Jul;9(7):979-92. doi: 10.1007/BF00964528.
Severe hypoglycemia with isoelectric EEG induced extensive deterioration of the energy state and gross alteration of amino acid contents on the rat cerebral and cerebellar cortex. During recovery, tissue glucose concentration returned to normal, while both lactate and pyruvate concentrations increased to above normal. In the recovery period, the ATP concentration increased but the adenine nucleotide pool remained reduced, even if the ADP and AMP contents were close to normal. Phosphocreatine was restored to normal concentration with reciprocal changes in creatine content. During recovery there was a rise in glutamate and glutamine concentrations, gamma-aminobutyrate content returning to normal value. Ammonia and aspartate decreased below normal, while alanine increased above normal. The effect of some pharmacological agents on the posthypoglycemic recovery was tested: (a) Ergot alkaloids (dihydroergocristine, dihydroergocriptine, dihydroergocornine); (b) Vinca minor alkaloids (vincamine TPS, (-) eburnamonine); (c) Rauwolfia serpentina alkaloids (reserpine, raubasine); (d) synthetic agent (piracetam). During the posthypoglycemic recovery, these different agents exhibited different, or even contrasting, interferences on glycolytic metabolites, amino acids and energy-rich phosphates. The metabolic alterations in the cerebellar cortex were qualitatively of the same character of those in neocortex. However, the metabolic alterations were less extensive and more sensitive to drug action.
伴有脑电图等电位的严重低血糖导致大鼠大脑和小脑皮质能量状态广泛恶化以及氨基酸含量显著改变。在恢复过程中，组织葡萄糖浓度恢复正常，而乳酸和丙酮酸浓度升高至正常水平以上。在恢复期，ATP浓度升高，但腺嘌呤核苷酸池仍减少，即使ADP和AMP含量接近正常。磷酸肌酸恢复到正常浓度，同时肌酸含量发生相应变化。在恢复过程中，谷氨酸和谷氨酰胺浓度升高，γ-氨基丁酸含量恢复到正常值。氨和天冬氨酸降至正常水平以下，而丙氨酸升至正常水平以上。测试了一些药理剂对低血糖后恢复的影响：(a)麦角生物碱（双氢麦角克碱、双氢麦角隐亭、双氢麦角柯宁碱）；(b)长春花生物碱（长春胺TPS、(-)长春胺）；(c)蛇根木生物碱（利血平、萝巴新）；(d)合成药剂（吡拉西坦）。在低血糖后恢复过程中，这些不同的药剂对糖酵解代谢物、氨基酸和富含能量的磷酸盐表现出不同甚至相反的干扰作用。小脑皮质的代谢改变在性质上与新皮质相同。然而，代谢改变范围较小，且对药物作用更敏感。
