Sasaki T, Kassell N F, Turner D M, Yamashita M, Spallone A
Neurosurgery. 1984 Nov;15(5):683-9. doi: 10.1227/00006123-198411000-00008.
The purpose of this study was to develop an inexpensive experimental model of carotid endarterectomy. Unilateral common carotid endarterectomies 3 mm in length were performed on 25 rats using microsurgical techniques. The pattern of endothelial regrowth after endarterectomy, the pinocytosis of regenerated endothelial cells, and the effects of heparin and thromboxane A2 synthetase inhibitor on thrombus formation were examined 7 days after the resumption of flow. Pinocytotic vesicular transport in endothelial cells was evaluated using horseradish peroxidase. All endarterectomized vessels in both control and drug-treated groups were patent 7 days after operation without apparent mural thrombus formation. Regenerated endothelial cells had spindle-shaped nuclei and cell bodies with long axes oriented parallel to the direction of blood flow. There were several instances where the leading edge of the endothelium at the distal end was reflected back in the distal direction. The extent of the endothelial ingrowth was often greater from the proximal end of the endarterectomy than from the distal end. These observations suggest that hemodynamic force affects endothelial regeneration. Pinocytotic activity was less in the regenerated endothelium than in either the endothelium remaining at the periphery of the endarterectomy or the endothelium of the control animals.
本研究的目的是建立一种廉价的颈动脉内膜切除术实验模型。使用显微外科技术对25只大鼠进行了长度为3毫米的单侧颈总动脉内膜切除术。在恢复血流7天后，检查了内膜切除术后内皮再生的模式、再生内皮细胞的胞饮作用以及肝素和血栓素A2合成酶抑制剂对血栓形成的影响。使用辣根过氧化物酶评估内皮细胞中的胞饮小泡运输。对照组和药物治疗组中所有接受内膜切除术的血管在术后7天均保持通畅，无明显壁血栓形成。再生内皮细胞具有梭形细胞核和细胞体，其长轴与血流方向平行。有几例远端内皮前缘向远端折返的情况。内膜切除术近端的内皮向内生长的程度通常比远端更大。这些观察结果表明血流动力学力影响内皮再生。再生内皮中的胞饮活性低于保留在内膜切除术周边的内皮或对照动物的内皮。
