Ryl'tsev V V, Vlasov L G, Samoĭlova T I, Volkovinskaia L P, Bondareva L N
Prikl Biokhim Mikrobiol. 1984 Sep-Oct;20(5):694-8.
The effect of ionizing radiation of 0.05-10 Mrad on trypsin immobilized on dialdehyde cellulose was being studied. After irradiation the activity of native trypsin decreases by 25%, as compared with the initial, while the activity of immobilized trypsin remains constant. Before immobilization cellulose undergoes special pretreatment that leads to a decrease in the initial contamination. Some samples of modified cellulose were contaminated by staphylococcus culture (200,000 microbes per 0.2 g) and then exposed to irradiation of 0.05-0.4 Mrad. A distinct correlation between the irradiation dose (0.05-0.4 Mrad) and contamination of the object was registered.
正在研究0.05 - 10兆拉德的电离辐射对固定在二醛纤维素上的胰蛋白酶的影响。辐照后，天然胰蛋白酶的活性与初始活性相比降低了25%，而固定化胰蛋白酶的活性保持不变。在固定化之前，纤维素经过特殊预处理，这导致初始污染减少。一些改性纤维素样品被葡萄球菌培养物污染（每0.2克含200,000个微生物），然后接受0.05 - 0.4兆拉德的辐照。记录到辐照剂量（0.05 - 0.4兆拉德）与物体污染之间存在明显的相关性。
