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[体外或体内肉瘤37细胞类核的修复。HEPES缓冲液的作用]

[Repair of the nucleoid of sarcoma 37 cells in vitro or in vivo. The effect of HEPES buffer].

作者信息

Synzynys B I, Trofimova S F

出版信息

Radiobiologiia. 1984 Sep-Oct;24(5):641-2.

PMID:6505155
Abstract

The method of sedimentation was used to study the repair of Sarcoma 37 cell nucleoid after irradiation of cells in vitro or after irradiation of mice with a dose of 9 Gy. It was shown that the process of repair in vitro was completed after 10 min of incubation of cells in the nutrient medium while the repair in vivo ceased 120 min after irradiation. In the presence of HEPES buffer (20 mM) in the medium the kinetics of the nucleoid repair in cells incubated in vitro was the same as that in vivo.

摘要

采用沉降法研究了体外照射细胞或给小鼠照射9 Gy剂量后肉瘤37细胞核仁的修复情况。结果表明,体外修复过程在细胞于营养培养基中孵育10分钟后完成,而体内修复在照射后120分钟停止。在培养基中存在HEPES缓冲液(20 mM)的情况下,体外孵育细胞的核仁修复动力学与体内相同。

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