Krarup T, Holst J J
Regul Pept. 1984 Sep;9(1-2):35-46. doi: 10.1016/0167-0115(84)90005-3.
The Sephadex G-50 gel filtration profile of immunoreactive gastric inhibitory polypeptide (GIP) in porcine and human gastrointestinal mucosa was determined in assays with antisera obtained from five different groups working with GIP. Tissue was extracted in acid ethanol or using a boiling method. Three well-defined components were detected with three of the antisera: one component corresponding to natural porcine GIP (5 kDa GIP), one component corresponding to what has been called 8 kDa GIP, and one component somewhat larger than the latter. One antiserum did not measure 8 kDa GIP at all, while the fifth antiserum measured small amounts of 8 kDa GIP in porcine but not in human extracts. While the antisera measured the same amounts of GIP in porcine extracts, two of the antisera measured significantly more GIP than the remaining three in extracts of human mucosa. The elution position of human 5 kDa GIP differed significantly from that of porcine 5 kDa GIP. In addition to the identification of a new molecular form of GIP, and the demonstration of important specificity differences among GIP antisera currently in use, the present results indicate that human and porcine 5 kDa GIP differ in chemical composition.
利用从五个研究胃肠抑制多肽（GIP）的不同小组获得的抗血清，测定了猪和人胃肠道黏膜中免疫反应性GIP的葡聚糖凝胶G - 50凝胶过滤图谱。组织用酸性乙醇提取或采用煮沸法提取。三种抗血清检测到三个明确的组分：一个组分对应天然猪GIP（5 kDa GIP），一个组分对应所谓的8 kDa GIP，还有一个组分比后者稍大。一种抗血清根本检测不到8 kDa GIP，而第五种抗血清在猪提取物中检测到少量8 kDa GIP，但在人提取物中未检测到。虽然抗血清在猪提取物中检测到的GIP量相同，但其中两种抗血清在人黏膜提取物中检测到的GIP明显多于其余三种。人5 kDa GIP的洗脱位置与猪5 kDa GIP的洗脱位置有显著差异。除了鉴定出一种新的GIP分子形式，并证明目前使用的GIP抗血清之间存在重要的特异性差异外，目前的结果还表明，人和猪的5 kDa GIP在化学组成上存在差异。
