Kohl N E, Gee C E, Alt F W
Science. 1984 Dec 14;226(4680):1335-7. doi: 10.1126/science.6505694.
In neuroblastoma lines in which the N-myc gene is present as a single copy, the expression of N-myc as messenger RNA is increased relative to that in nonneuroblastoma cell lines and tumors. The increase of expression in neuroblastomas with amplified N-myc genes is the result of (i) an increase in the absolute amount of expression of each N-myc gene and (ii) an increase in the copy number of the N-myc gene. A second gene--which is amplified in many of the same lines as N-myc--is expressed to about the same degree in most human cell lines and primary tumors regardless of origin (when normalized to gene copy number). Thus, a change in the regulation of N-myc expression in neuroblastomas and certain other tumors results in greatly increased expression of each N-myc gene copy.
在N - myc基因以单拷贝形式存在的神经母细胞瘤细胞系中，相对于非神经母细胞瘤细胞系和肿瘤，N - myc作为信使RNA的表达增加。在N - myc基因扩增的神经母细胞瘤中，表达增加是以下两个因素的结果：（i）每个N - myc基因表达的绝对量增加；（ii）N - myc基因拷贝数增加。另一个基因——在许多与N - myc相同的细胞系中扩增——在大多数人类细胞系和原发性肿瘤中，无论其来源如何（当按照基因拷贝数进行标准化时），表达程度大致相同。因此，神经母细胞瘤和某些其他肿瘤中N - myc表达调控的改变导致每个N - myc基因拷贝的表达大幅增加。
