Hagan R C, Leszczynski D E, Kummerow F A
Toxicol Appl Pharmacol. 1984 Dec;76(3):483-9. doi: 10.1016/0041-008x(84)90352-1.
The effects of estradiol and progesterone treatment on plasma hormone and lipid concentrations were measured in laying hens and sexually immature pullets. Pullets and hens were divided into three groups and injected with estradiol (1 mg/kg bw), progesterone (4 mg/kg bw), or vehicle (propylene glycol) once each day for 14 days. Blood samples were collected before treatment and 24 hr after the 7th and 14th treatments. Plasma progesterone (P), estradiol (E2), triglycerides (TG), cholesterol (C), and phospholipids (PL) were measured. E2 treatments elevated hen plasma TG 7.2X, PL 5.1X, and C 7.2X; and pullet plasma TG 6.8X, PL 3.7X, and C 2.5X. However, because hen plasma was initially mildly hyperlipidemic, the E2-treated hens developed severe hyperlipidemia, but egg production was unaffected. Progesterone treatments of pullets had little or no effect on plasma lipids, but progesterone treatment of hens significantly reduced initial plasma TG and PL and also reduced egg production. No substantial differences were found in circulating E2 or P in hormone-treated hens and pullets, which indicated no extreme differences in plasma hormone clearance rates. These results indicate that long-term rather than short-term hepatic priming may account for observed differences in layer and pullet response to estradiol treatment.
在产蛋母鸡和性未成熟小母鸡中测量了雌二醇和孕酮处理对血浆激素和脂质浓度的影响。将小母鸡和母鸡分为三组，每天注射一次雌二醇（1毫克/千克体重）、孕酮（4毫克/千克体重）或赋形剂（丙二醇），持续14天。在处理前以及第7次和第14次处理后24小时采集血样。测量血浆孕酮（P）、雌二醇（E2）、甘油三酯（TG）、胆固醇（C）和磷脂（PL）。E2处理使母鸡血浆TG升高7.2倍、PL升高5.1倍、C升高7.2倍；使小母鸡血浆TG升高6.8倍、PL升高3.7倍、C升高2.5倍。然而，由于母鸡血浆最初轻度高脂血症，经E2处理的母鸡出现严重高脂血症，但产蛋不受影响。孕酮处理小母鸡对血浆脂质几乎没有影响，但孕酮处理母鸡显著降低了初始血浆TG和PL，也降低了产蛋量。在激素处理的母鸡和小母鸡中，循环E2或P未发现实质性差异，这表明血浆激素清除率没有极端差异。这些结果表明，长期而非短期的肝脏启动可能是观察到的蛋鸡和小母鸡对雌二醇处理反应差异的原因。
