Jeje M O, Blajchman M A, Steeves K, Horsewood P, Kelton J G
Transfusion. 1984 Nov-Dec;24(6):473-6. doi: 10.1046/j.1537-2995.1984.24685066803.x.
In this report, we describe a sensitive immunoradiometric assay (IRMA) for quantitating IgG on the surface of red cells. Washed red cells were prepared to a purity of greater than 99.9 percent. Varying dilutions of these cells were incubated with a fixed concentration of 125I-anti-IgG. After equilibrium was achieved, the unbound 125I-anti-IgG was measured by the addition of IgG covalently linked to agarose beads. The red cells were lysed by detergent, and the 125I-anti-IgG bound to the IgG-beads was measured. The amount of IgG on the red cells was determined by relating the concentration of test red cells causing 50 percent inhibition of binding of the 125I-anti-IgG to the IgG-beads to 50 percent inhibition of binding caused by the IgG standard. Using this assay, the red cell-associated IgG (RCA-IgG) of 20 healthy male and female controls with normal hemoglobin concentrations was 7.23 +/- 6.11 fg IgG per 10(3) cells (mean +/- 2 SD). The mean RCA-IgG on washed cells from 34 different tests performed on 19 anemic patients with clinically diagnosed autoimmune hemolytic anemia was 176.1 +/- 375.6 fg IgG per 10(3) cells. There was no correlation between the levels of RCA-IgG and the hemoglobin levels or reticulocyte counts in these patients.
在本报告中，我们描述了一种用于定量红细胞表面IgG的灵敏免疫放射分析（IRMA）方法。制备的洗涤红细胞纯度大于99.9%。将这些细胞的不同稀释液与固定浓度的125I-抗IgG孵育。达到平衡后，通过加入与琼脂糖珠共价连接的IgG来测量未结合的125I-抗IgG。用去污剂裂解红细胞，并测量与IgG珠结合的125I-抗IgG。通过将导致125I-抗IgG与IgG珠结合抑制50%的测试红细胞浓度与IgG标准品引起的结合抑制50%相关联，来确定红细胞上IgG的量。使用该分析方法，20名血红蛋白浓度正常的健康男性和女性对照的红细胞相关IgG（RCA-IgG）为每10(3)个细胞7.23±6.11 fg IgG（平均值±2标准差）。对19例临床诊断为自身免疫性溶血性贫血的贫血患者进行的34次不同测试中，洗涤细胞上的平均RCA-IgG为每10(3)个细胞176.1±375.6 fg IgG。这些患者的RCA-IgG水平与血红蛋白水平或网织红细胞计数之间无相关性。
