Bassøe C F
Acta Pathol Microbiol Immunol Scand C. 1984 Jun;92(3):167-71. doi: 10.1111/j.1699-0463.1984.tb00069.x.
Polymorphonuclear neutrophilic leukocyte (PMNL) phagocytosis of fluorescein-isothiocyanate labelled Staphylococcus aureus was studied by flow cytometry (FCM) in 41 patients with bacterial infections. Practically all phagocytic cells (granulocytes and monocytes) showed active uptake of fluorescent bacteria. However, the mean fluorescence of the phagocytes was about 15% lower than that of cells from healthy volunteers. This reduction was only seen in the male patients, not in the females. The fluorescence reduction was related neither to the type of infectious agent (Gram-positive versus Gram-negative) nor to the white blood cell count or percentage of immature leukocytes. Five patients with cured infections had phagocyte fluorescence similar to that of the controls. In two patients, phagocyte fluorescence was very low, indicating reduced phagocytosis. Phagocyte fluorescence was not related to patient survival.
采用流式细胞术（FCM）对41例细菌感染患者的多形核嗜中性白细胞（PMNL）吞噬异硫氰酸荧光素标记的金黄色葡萄球菌的情况进行了研究。实际上所有吞噬细胞（粒细胞和单核细胞）均表现出对荧光细菌的主动摄取。然而，吞噬细胞的平均荧光比健康志愿者细胞的平均荧光低约15%。这种降低仅在男性患者中出现，女性患者未出现。荧光降低既与感染病原体的类型（革兰氏阳性与革兰氏阴性）无关，也与白细胞计数或未成熟白细胞百分比无关。5例感染已治愈的患者的吞噬细胞荧光与对照组相似。2例患者的吞噬细胞荧光非常低，表明吞噬作用降低。吞噬细胞荧光与患者生存率无关。
