Simon M R, Holmes K R, Olsen A M
Anat Rec. 1984 Oct;210(2):333-41. doi: 10.1002/ar.1092100208.
The purpose of this investigation was to subject separate male and female groups of weanling hypophysectomized rats each to a specific 10% simulated increase in body weight using constant centrifugation, ranging from 1.1G to 2.0G, to study changes in bone robusticity and bone mineral content. (In this paper, "G" is the acceleration due to gravity.) After 60 days of centrifugation at 24 rpm, the rats were killed and the humerus, radius, ulna, femur, and tibia were removed, cleared of soft tissues, weighed, measured, decalcified with EDTA, and reweighed. Bone robusticity was determined using the ponderal index: P.I. = bone length divided by 3 square root bone weight; and bone mineral content (BMC) was determined using the formula: BMC = [(Wu - Wd) divided by Wu] X 100. Tukey's Studentized Multiple Range T Test was used. The data suggest that, for both male and female hypophysectomized rats, bone robusticity is decreased with simulated increases in body weight; also, for males, a bimodal curve describing the relationship between BMC and simulated increases in body weight is suggested.
本研究的目的是使用从1.1G到2.0G的恒定离心力，对雄性和雌性断乳垂体切除大鼠单独分组，使每组大鼠体重模拟增加10%，以研究骨强度和骨矿物质含量的变化。（在本文中，“G”是重力加速度。）以24转/分钟的速度离心60天后，处死大鼠，取出肱骨、桡骨、尺骨、股骨和胫骨，清除软组织，称重、测量，用乙二胺四乙酸（EDTA）脱钙，然后再次称重。使用重量指数确定骨强度：重量指数=骨长度除以3倍骨重量的平方根；使用公式确定骨矿物质含量（BMC）：BMC = [（Wu - Wd）除以Wu]×100。采用Tukey氏学生化多重极差T检验。数据表明，对于雄性和雌性垂体切除大鼠，随着体重模拟增加，骨强度降低；此外，对于雄性大鼠，提示了一条描述BMC与体重模拟增加之间关系的双峰曲线。
