Pichinoty F, Asselineau J
Ann Microbiol (Paris). 1984 Sep-Oct;135B(2):199-207.
When cultivated on solid medium or in stationary liquid medium, most of the 11 studied strains of Bacillus benzoevorans grew as unbranched, flexible, immotile filaments (or trichomes) of undefined length and 1.8 micrometer diameter. They were enclosed in a sheath giving an overall diameter of 3.6 micrometer. When cultivated in vigorously shaken liquid medium, several strains grew as separate rods (1.8 X 2.2 micrometer), did not deform the filament and contained dipicolinic acid. The cytoplasm had a granular aspect due to the presence of poly-beta-hydroxybutyric acid. The Gram reaction was variable but the Gram type was positive. Isolated and purified sheath contained 73% proteins, 12.9% reducing sugars and 8% lipids. In stationary liquid medium, the culture had a mycelial aspect and a thick pellicle formed at the surface. Colonies were circular, flat, opaque, whitish, mat and compact; they had irregular edges, spread out on the surface and did not adhere to the agar.
当在固体培养基或静止液体培养基中培养时,11株研究的苯食芽孢杆菌大多生长为长度不定、直径1.8微米的无分支、柔韧、不运动的丝状体(或藻丝体)。它们被包裹在一个鞘中,整体直径为3.6微米。当在剧烈振荡的液体培养基中培养时,一些菌株生长为单独的杆菌(1.8×2.2微米),不使丝状体变形,且含有吡啶二羧酸。由于存在聚-β-羟基丁酸,细胞质呈现颗粒状外观。革兰氏反应可变,但革兰氏类型为阳性。分离纯化的鞘含有73%的蛋白质、12.9%的还原糖和8%的脂质。在静止液体培养基中,培养物呈菌丝状外观,表面形成一层厚厚的菌膜。菌落圆形、扁平、不透明、白色、毡状且致密;边缘不规则,在表面扩散,不粘附于琼脂。