Pichinoty F, Asselineau J, Mandel M
Ann Microbiol (Paris). 1984 Sep-Oct;135B(2):209-17.
The eleven strains studied were prototrophic and did not grow in media containing only 1% Bacto-peptone or Bacto-tryptone; they grew rapidly in media containing 0.4% yeast extract and 0.2% sodium acetate or benzoate. The maximal growth temperature ranged from 39 to 45 degrees C. Six aliphatic acids, four aromatic acids and five phenols were used as sole carbon and energy sources by the 11 strains. Carbohydrates and amino acids (except for glycine) were not used as carbon and energy sources. Nitrate (but not nitrite) was used anaerobically as a respiratory electron acceptor. Nitrous oxide was used and reduced to N2 by only 3 strains. The mean guanine-plus-cytosine content of the DNA was 41.3 +/- 1.1 mol %. Morphologically and nutritionally, the bacteria described are clearly different from the 5 known species of the first morphological group whose cells have a diameter greater than 1 micrometer: Bacillus megaterium, B. cereus, B. cereus var. mycoides, B. macroides, B. badius, and B. fastidiosus. Strain B1 (=CCM 3364) is the holotype of Bacillus benzoevorans sp. nov.
所研究的11个菌株是原养型的,在仅含有1%细菌蛋白胨或胰蛋白胨的培养基中不能生长;它们在含有0.4%酵母提取物和0.2%醋酸钠或苯甲酸钠的培养基中生长迅速。最高生长温度范围为39至45摄氏度。11个菌株将6种脂肪酸、4种芳香酸和5种酚类用作唯一的碳源和能源。碳水化合物和氨基酸(除甘氨酸外)未被用作碳源和能源。硝酸盐(而非亚硝酸盐)在厌氧条件下用作呼吸电子受体。只有3个菌株利用并将一氧化二氮还原为N2。DNA的鸟嘌呤加胞嘧啶平均含量为41.3±1.1摩尔%。在形态和营养方面,所描述的细菌与第一形态组中已知的5个物种明显不同,后者细胞直径大于1微米,分别为巨大芽孢杆菌、蜡样芽孢杆菌、蜡样芽孢杆菌蕈状变种、大芽孢杆菌、浅黄芽孢杆菌和苛求芽孢杆菌。菌株B1(=CCM 3364)是苯甲酸芽孢杆菌新种的模式菌株。