红细胞磷酸己糖途径速率与6-磷酸葡萄糖浓度之间的关系。

Relationship between the rate of erythrocyte hexose monophosphate pathway and the glucose 6-phosphate concentration.

作者信息

Magnani M, Stocchi V, Fazi A, Dachà M, Fornaini G

出版信息

Biochem Biophys Res Commun. 1984 Nov 30;125(1):14-7. doi: 10.1016/s0006-291x(84)80326-5.

Abstract

Erythrocytes of individuals with increased (+ 50%) or reduced (-35%) hexokinase activity contain respectively 70 and 17 nmole/ml RBC of glucose-6-phosphate (normal concentration 30 +/- 5nmole/ml RBC) and show comparable rates of the HMP (60 +/- 5nmole/hr/ml RBC). Similarly, in RBC of different ages, obtained by density gradient ultracentrifugation, the glucose-6-phosphate concentration range from 57 (young cells) to 18 (old cells) nmole/ml RBC but the rate at which glucose is utilized in the HMP is unchanged. These data exclude a regulatory role of glucose 6-phosphate in the HMP even if its concentration is under that required for maximal G6PD activity.

摘要

己糖激酶活性增加（+50%）或降低（-35%）的个体的红细胞，分别含有70和17纳摩尔/毫升红细胞的6-磷酸葡萄糖（正常浓度为30±5纳摩尔/毫升红细胞），并且显示出相当的磷酸戊糖途径速率（60±5纳摩尔/小时/毫升红细胞）。同样，通过密度梯度超速离心获得的不同年龄的红细胞中，6-磷酸葡萄糖浓度范围为57（年轻细胞）至18（衰老细胞）纳摩尔/毫升红细胞，但磷酸戊糖途径中葡萄糖的利用速率未改变。这些数据排除了6-磷酸葡萄糖在磷酸戊糖途径中的调节作用，即使其浓度低于最大葡萄糖-6-磷酸脱氢酶活性所需的浓度。