抗坏血酸与酒精氧化

Ascorbic acid and alcohol oxidation.

作者信息

Susick R L, Zannoni V G

出版信息

Biochem Pharmacol. 1984 Dec 15;33(24):3963-9. doi: 10.1016/0006-2952(84)90009-1.

Abstract

Methanol and ethanol were rapidly metabolized to formaldehyde and acetaldehyde in the presence of ascorbate, 1,10-phenanthroline and either guinea pig hepatic 100,000 g supernatant or 12,000 g pellet fractions. The specific activity of methanol oxidation was 1720 nmoles formaldehyde formed/min/mg protein in the 100,000 g fraction and 790 in the 12,000 g pellet fraction. The specific activity of ethanol oxidation was 1590 nmoles acetaldehyde formed/min/mg protein in the 100,000 g fraction and 820 in the 12,000 g pellet fraction. The activity was enzymatic in that it was linear with time, proportional to protein concentration, and sensitive to temperature. Catalase appeared to be the enzymatic component responsible for the oxidation. In this ascorbate-dependent alcohol oxidation system, oxygen was consumed and H2O2 was formed. When purified catalase and ascorbate were used, complex I was detected and methanol was oxidized.

摘要

在抗坏血酸盐、1,10 - 菲啰啉以及豚鼠肝脏100,000g上清液或12,000g沉淀组分存在的情况下，甲醇和乙醇迅速代谢为甲醛和乙醛。甲醇氧化的比活性在100,000g组分中为每分钟每毫克蛋白质形成1720纳摩尔甲醛，在12,000g沉淀组分中为790。乙醇氧化的比活性在100,000g组分中为每分钟每毫克蛋白质形成1590纳摩尔乙醛，在12,000g沉淀组分中为820。该活性具有酶促性质，即它随时间呈线性，与蛋白质浓度成正比，且对温度敏感。过氧化氢酶似乎是负责氧化的酶组分。在这个依赖抗坏血酸盐的醇氧化系统中，消耗了氧气并形成了过氧化氢。当使用纯化的过氧化氢酶和抗坏血酸盐时，检测到复合物I且甲醇被氧化。