Vezzadini P, Bonora G, Tomassetti P, Favaro A, Toni R, Ecchia R, Labò G
Boll Ist Sieroter Milan. 1984 Jul 31;63(3):201-6.
A method for measurement of gastrin in human antral mucosa or in extragastric tissue has been developed and validated. Tissue gastrin was extracted by boiling followed by homogenization at neutral pH. Extractable gastrin immunoreactivity was measured by radioimmunoassay using an antiserum with equal affinity towards G-17 I, G-17 II, G-34 I and G-34 II molecular forms. Almost all extractable gastrin immunoreactivity was recovered after a single extraction and no significant interference by other peptides and/or substances present in tissue was found. The mean gastrin concentration in antral mucosa of healthy subjects was similar to that observed in duodenal ulcer patients, while patients with type A chronic atrophic gastritis or with antral gastrin cell hyperplasia had mean values significantly higher. Gastrin concentration in all specimens from gastrinoma or its metastases was above the upper limit of the range of control tissue. Measurement of tissue gastrin seems to be a valuable tool in the diagnosis of antral gastrin cell hyperplasia and Zollinger-Ellison syndrome.
一种用于测量人胃窦黏膜或胃外组织中胃泌素的方法已得到开发和验证。通过煮沸然后在中性pH下匀浆来提取组织胃泌素。使用对G-17 I、G-17 II、G-34 I和G-34 II分子形式具有同等亲和力的抗血清，通过放射免疫测定法测量可提取的胃泌素免疫反应性。单次提取后几乎所有可提取的胃泌素免疫反应性都能回收，且未发现组织中存在的其他肽和/或物质有明显干扰。健康受试者胃窦黏膜中的胃泌素平均浓度与十二指肠溃疡患者中观察到的相似，而A型慢性萎缩性胃炎患者或胃窦胃泌素细胞增生患者的平均值明显更高。胃泌素瘤或其转移灶的所有标本中的胃泌素浓度均高于对照组织范围的上限。组织胃泌素的测量似乎是诊断胃窦胃泌素细胞增生和佐林格-埃利森综合征的有价值工具。
