Wang P Y, Youson J H, Drakos T T
Biochim Biophys Acta. 1984 Dec 20;802(3):467-76. doi: 10.1016/0304-4165(84)90366-0.
In a previous study of the dextran gel sphere model system, a possible correlation between cell deformability and agglutinability by concanavalin A was indicated. Cell deformability was evaluated as filtrability, using polycarbonate membrane filtration. With 25-mm diameter filters and 5-ml cell suspensions at (0.8-16) X 10(5) cells/ml, the filtrability at a given filter pore size was highly reproducible and was not affected by variations in cell population, viability, washings of cells retained on filter, or temperature. The filtrability of EDTA-dissociated 3T3 cells through 12-micron pore size filter was 8%, and a suspension of 10(6) cells/ml was not agglutinated by 600 micrograms concanavalin A. The filtrability of trypsin-dissociated 3T3 cells was 95%, and these cells were agglutinated by 200 micrograms of the lectin. EDTA-dissociated SV-3T3 cells had a filtrability of 73% and were also highly agglutinable. Formalin fixation reduced the high filtrability to 6%, and also abolished the agglutinability. As a further test of the correlation, trypsin-dissociated 3T3 cells were admixed with the fixed cells. The agglutinability varied with the proportions of the two cell components, and the admixtures could be separated according to filtrability into the original components with distinctly different agglutinability. Furthermore, 25% of a random population of EDTA-dissociated SV-3T3 cells retained by the filter were found to be non-agglutinable. The separated SV-3T3 cell fractions could also form admixtures of different agglutinability. It is concluded that the agglutinability of mouse 3T3 and SV-3T3 cells by concanavalin A can be correlated with and predicted by cell filtrability.
在先前一项关于葡聚糖凝胶球模型系统的研究中，表明细胞可变形性与伴刀豆球蛋白A介导的凝集性之间可能存在关联。细胞可变形性通过聚碳酸酯膜过滤法评估为过滤性。使用直径25毫米的滤器和浓度为(0.8 - 16)×10⁵个细胞/毫升的5毫升细胞悬液，在给定滤器孔径下的过滤性具有高度可重复性，且不受细胞群体、活力、滤器上留存细胞的洗涤或温度变化的影响。经乙二胺四乙酸（EDTA）解离的3T3细胞通过12微米孔径滤器的过滤性为8%，每毫升10⁶个细胞的悬液不会被600微克伴刀豆球蛋白A凝集。经胰蛋白酶解离的3T3细胞的过滤性为95%，这些细胞会被200微克该凝集素凝集。经EDTA解离的SV - 3T3细胞的过滤性为73%，且也具有高度凝集性。福尔马林固定将高过滤性降低至6%，同时也消除了凝集性。作为对这种相关性的进一步测试，将经胰蛋白酶解离的3T3细胞与固定细胞混合。凝集性随两种细胞成分的比例而变化，并且混合物可根据过滤性分离为具有明显不同凝集性的原始成分。此外，发现被滤器留存的经EDTA解离的SV - 3T3细胞随机群体中有25%是不可凝集的。分离出的SV - 3T3细胞组分也可形成具有不同凝集性的混合物。得出的结论是，伴刀豆球蛋白A对小鼠3T3和SV - 3T3细胞的凝集性可与细胞过滤性相关联并由其预测。
