Kohen E, Kohen C, Reyftmann J P, Morliere P, Santus R
Biochim Biophys Acta. 1984 Dec 11;805(4):332-6. doi: 10.1016/0167-4889(84)90015-6.
Microspectrofluorometry of L and WI-38 cells reveals chemical/structural changes due to quiescence or senescence, i.e., lipid peroxidation, spontaneous or photosensitized by hematoporphyrin. Cells treated with hematoporphyrin and a lysosomal umbelliferone probe show a fast-rising umbelliferone emission, plus a fluorescent photoproduct. Studies in rapidly growing versus quiescent L, early passage/late passage WI-38 cells, suggest accumulation of fluorescence Schiff bases (i.e., their association with granular regions of cells in stationary phase, spectral properties, fast increase in photosensitized cells) and a possible lysosomal membrane permeabilization in quiescent or senescent cells.
对L细胞和WI - 38细胞进行显微分光荧光测定，揭示了由于静止或衰老引起的化学/结构变化，即脂质过氧化，包括自发的或由血卟啉光敏化的脂质过氧化。用卟啉和溶酶体伞形酮探针处理的细胞显示伞形酮发射快速增加，以及一种荧光光产物。对快速生长的L细胞与静止的L细胞、早期传代/晚期传代的WI - 38细胞的研究表明，荧光席夫碱会积累（即它们与静止期细胞的颗粒区域相关、光谱特性、光敏化细胞中快速增加），并且在静止或衰老细胞中可能存在溶酶体膜通透性增加的情况。
