Dolezel S, Shenderov S M, Törökova R
Biull Eksp Biol Med. 1984 Nov;98(11):630-1.
A modification of Pickworth's method for detection of open capillaries was used. The modification is based on freezing an organ in situ with liquid propane, cooled with liquid nitrogen, followed by lyophilization of the preparation and its fixation in gaseous formaldehyde. It was shown in rat experiments that the number of functioning capillaries in m. gastrocnemius and m. soleus 14 and 90-12 days after reduction of pressure in the hindquarter of the body with the narrowing of the abdominal aorta lumen was practically the same as that in the control animals. The data support the view that reduction of the hydraulic resistance in the area of regional hypotension does not correlate with increase in the number of parallel-functioning vessels.
采用了一种对皮克沃思检测开放毛细血管方法的改良方法。该改良方法基于用液氮冷却的液态丙烷原位冷冻器官,随后对制剂进行冻干并在气态甲醛中固定。大鼠实验表明,在腹主动脉管腔狭窄导致身体后肢减压后14天以及90 - 12天,腓肠肌和比目鱼肌中功能正常的毛细血管数量与对照动物的实际相同。这些数据支持了这样一种观点,即局部低血压区域液压阻力的降低与并行功能血管数量的增加并无关联。