通过放射自显影法定量测定大鼠脑和垂体中的血管紧张素II结合位点。
Quantitative determination of angiotensin II binding sites in rat brain and pituitary gland by autoradiography.
作者信息
Israel A, Correa F M, Niwa M, Saavedra J M
出版信息
Brain Res. 1984 Nov 26;322(2):341-5. doi: 10.1016/0006-8993(84)90130-6.
PMID:6509323
Abstract
Rat brain and pituitary angiotensin II (AII) binding sites were quantitated by incubation of tissue sections with 125I-[Sar1] AII, Ultrofilm radioautography, computerized densitometry, and comparison with 125I-standards at appropriate film exposure times. The highest number of AII binding sites was found in anterior pituitary and the circumventricular organs, organon subfornicalis and organon vasculosum laminae terminalis.
摘要
通过将组织切片与125I-[Sar1]血管紧张素II（AII）一起孵育、使用超薄膜放射自显影术、计算机化密度测定法，并在适当的胶片曝光时间与125I标准品进行比较，对大鼠脑和垂体中的AII结合位点进行了定量分析。发现AII结合位点数量最多的部位是垂体前叶和室周器官，即穹窿下器和终板血管器。
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