Yu B S, Jo I H
Chem Biol Interact. 1984 Dec;52(2):185-202. doi: 10.1016/0009-2797(84)90072-3.
The effect of three sea cucumber saponins, echinoside A, bivittoside D and holothurin A, on multilamellar liposomes was investigated. An ideal osmotic behavior of liposomes was described as a linear relationship between the reciprocal 3/2 s power of absorbance at 450 nm and the osmotic gradient across the membrane. Sea cucumber saponins at concentrations below critical micelle concentration (CMC) disturbed this linear relationship in liposomes composed of egg phosphatidylcholine, phosphatidic acid and cholesterol. Cholesterol-free liposomes were not susceptible to these saponins. Results of optical measurements were consistent with those of transmission electron microscopy, which showed saponin-induced changes in liposomal structure. The lytic activity of sea cucumber saponins on liposomes depended on their chemical structure. These results suggest that sea cucumber saponins as monomers can interact with liposomes and that cholesterol serve as a principal binding site for the sea cucumber saponins.
研究了三种海参皂苷，即海参苷A、双 vittoside D 和海参毒素A对多层脂质体的影响。脂质体的理想渗透行为被描述为 450 nm 处吸光度的倒数 3/2 次幂与跨膜渗透梯度之间的线性关系。低于临界胶束浓度（CMC）的海参皂苷扰乱了由卵磷脂、磷脂酸和胆固醇组成的脂质体中的这种线性关系。无胆固醇的脂质体对这些皂苷不敏感。光学测量结果与透射电子显微镜的结果一致，后者显示了皂苷诱导的脂质体结构变化。海参皂苷对脂质体的裂解活性取决于它们的化学结构。这些结果表明，作为单体的海参皂苷可以与脂质体相互作用，并且胆固醇是海参皂苷的主要结合位点。
