使用通过[3H]甲基交换在N端区域特异性标记的牛甲状旁腺激素，来比较该激素在大鼠体内的肝脏和肾脏代谢情况。

Use of bovine parathyroid hormone, labelled specifically in the N-terminal region by [3H]methyl exchange, to compare hepatic and renal metabolism of this hormone in the rat.

作者信息

Barling P M, Christie D L

出版信息

Comp Biochem Physiol B. 1984;79(3):435-40. doi: 10.1016/0305-0491(84)90401-2.

Abstract

[3H]bPTH, prepared by [3H]methyl exchange, was administered intravenously to rats. Analysis of kidney and liver extracts on Sephadex G-50 showed some low mol.wt (peak IV) and some bound (peak Ia) material. A peak of intermediate mol.wt (peak II) was present in kidney but absent from liver. Kidneys that had been perfused with [3H]bPTH contained similar metabolites to those described, indicating that they are generated de novo. Analysis of peak II by reverse-phase h.p.l.c. showed a number of metabolites. The significance of these findings is discussed. This study demonstrates the value of [3H]methyl exchange labelling in investigating peptide metabolism.

摘要

通过[³H]甲基交换制备的[³H]bPTH经静脉注射给大鼠。对肾和肝提取物在Sephadex G - 50上进行分析，结果显示出一些低分子量物质（峰IV）和一些结合物质（峰Ia）。肾中存在一个中等分子量的峰（峰II），而肝中没有。用[³H]bPTH灌注过的肾含有与上述相似的代谢产物，这表明它们是重新生成的。通过反相高效液相色谱法对峰II进行分析，结果显示有多种代谢产物。文中讨论了这些发现的意义。本研究证明了[³H]甲基交换标记在研究肽代谢中的价值。