东北大西洋鱼类物种组织中的氧化三甲胺酶（TMAO-ase）活性。

TMAO-ase activity in tissues of fish species from the Northeast Atlantic.

作者信息

Rehbein H, Schreiber W

出版信息

Comp Biochem Physiol B. 1984;79(3):447-52. doi: 10.1016/0305-0491(84)90403-6.

Abstract

The influence of various parameters (evacuation; pH value; addition of Fe2+, cysteine, ascorbic acid and methylene blue) on the activity of the trimethylamine oxide demethylase (TMAO-ase) in extracts from the kidney of saithe (Pollachius virens L.) is described. Assay conditions resulting in maximum formaldehyde (FA) production were used to measure the TMAO-ase activity in kidney, spleen, liver, pyloric caeca, blood and muscle from Northern Atlantic fish species. Only in organs from fishes of the order Gadiformes could TMAO-ase activity be detected. The TMAO-ase was most active in kidney and spleen whereas in muscle the enzyme activity could be barely detected.

摘要

描述了各种参数（抽真空；pH值；添加Fe2+、半胱氨酸、抗坏血酸和亚甲基蓝）对绿青鳕（Pollachius virens L.）肾脏提取物中氧化三甲胺脱甲基酶（TMAO酶）活性的影响。采用能产生最大量甲醛（FA）的测定条件，来测量北大西洋鱼类肾脏、脾脏、肝脏、幽门盲囊、血液和肌肉中的TMAO酶活性。仅在鳕形目鱼类的器官中能检测到TMAO酶活性。TMAO酶在肾脏和脾脏中活性最高，而在肌肉中几乎检测不到该酶的活性。