Tsou H R, Fiala R R, Mowery P C, Bullock M W
J Antibiot (Tokyo). 1984 Nov;37(11):1382-7. doi: 10.7164/antibiotics.37.1382.
The biosynthesis of the spermidine and guanidino groups has been studied with carbon-14 and carbon-13 labeled intermediates. Arginine, citrulline and ornithine are incorporated in good efficiency. The guanidino group of arginine and the ureido group of citrulline both label the guanidino group on the hexose sugar. None of the ureido groups in the antibiotic was enriched. It is likely that citrulline is converted to arginine before use in the biosynthesis. Arginine, citrulline and ornithine are incorporated as the four carbon unit of spermidine. All of the labeled 5 carbon from ornithine or from citrulline appears adjacent to the secondary amine in the four carbon unit of spermidine. This would indicate that unbound putrescine is not an immediate precursor of spermidine.
已使用碳 - 14和碳 - 13标记的中间体对亚精胺和胍基的生物合成进行了研究。精氨酸、瓜氨酸和鸟氨酸的掺入效率良好。精氨酸的胍基和瓜氨酸的脲基均标记己糖上的胍基。抗生素中的脲基均未富集。瓜氨酸在用于生物合成之前很可能先转化为精氨酸。精氨酸、瓜氨酸和鸟氨酸作为亚精胺的四碳单元被掺入。来自鸟氨酸或瓜氨酸的所有标记的五碳都出现在亚精胺四碳单元仲胺的相邻位置。这表明未结合的腐胺不是亚精胺的直接前体。
