一种用于流感病毒包膜抗原的竞争性抑制放射免疫测定法。

A competitive-inhibition radioimmunoassay for influenza virus envelope antigens.

作者信息

Russ G, Styk B, Poláková K, Varecková E

出版信息

Acta Virol. 1976 Dec;20(6):460-5.

PMID:65118

Abstract

A double-antibody competitive-inhibition radioimmunoassay for influenza virus envelope antigens is described. A viral antigen preparation from influenza A virus recombinant MRC11 [antigenically identical to A/Port Chalmers/1/73 (H3N2)] consisting of haemagglutinin and neuraminidase was labelled with radioiodine. Rabbit antisera were allowed to react with the labelled antigen and the resultant antigen-antibody complexes were precipitated with the appropriate antiglobulin. The competitive-inhibition radioimmunoassay was very sensitive in elucidation of differences even among closely related influenza virus strains. Attempts have been made to eliminate neuraminidase from radioimmunoprecipitation in order to obtain a competitive-inhibition radioimmunoassay system for haemagglutinin alone.

摘要

本文描述了一种用于流感病毒包膜抗原的双抗体竞争抑制放射免疫测定法。从甲型流感病毒重组体MRC11（抗原性与A/查尔姆斯港/1/73（H3N2）相同）制备的包含血凝素和神经氨酸酶的病毒抗原制剂用放射性碘进行了标记。使兔抗血清与标记抗原反应，然后用适当的抗球蛋白沉淀所得的抗原-抗体复合物。这种竞争抑制放射免疫测定法在阐明即使是密切相关的流感病毒株之间的差异方面非常灵敏。已尝试从放射免疫沉淀中去除神经氨酸酶，以获得仅用于血凝素的竞争抑制放射免疫测定系统。

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