Martínez-Calva I, Campos-Apáez A, Rosales-Vega E, Mourelle M
J Appl Toxicol. 1984 Oct;4(5):270-2. doi: 10.1002/jat.2550040513.
The effect of pretreatment with vitamin E on membrane lipid alterations produced by the acute intoxication with CCl4 was studied. Rats were treated with an oral dose of CCl4 (0.4 g per 100 g body weight) and 24 h later the animals were sacrificed and liver plasma membranes isolated. After extraction, the membrane lipids were analysed by thin-layer chromatography and quantitated by densitometry. The phospholipid: protein ratio determined in plasma membranes of CCl4-treated rats was almost three-fold higher than that found in control animals. Sphingomyelin (Sph) and phospharidyl choline (PC) increased, while phosphatidyl ethanolamine (PE) decreased in the plasma membranes isolated from the CCl4-treated group. Animals pretreated for 7 days with a daily dose of vitamin E (200 IU per kg body weight) showed a lower increase in the phospholipid: protein ratio (two-fold) and the changes in Sph, PC and PE were lower. When the rats were pretreated with a higher dose of vitamin E (400 IU per kg body weight) for the same period, the lipid composition of plasma membrane was normal. These results indicate that vitamin E can protect against the alterations induced by CCl4 on the liver membranes. The protective action of vitamin E against CCl4 membrane damage is probably associated with its antioxidant properties.
研究了维生素E预处理对四氯化碳急性中毒所致膜脂改变的影响。给大鼠口服四氯化碳（每100克体重0.4克），24小时后处死动物并分离肝细胞膜。提取后，通过薄层色谱法分析膜脂并用密度测定法定量。四氯化碳处理组大鼠细胞膜中磷脂与蛋白质的比率几乎是对照组动物的三倍。从四氯化碳处理组分离的细胞膜中，鞘磷脂（Sph）和磷脂酰胆碱（PC）增加，而磷脂酰乙醇胺（PE）减少。每日给予维生素E（每千克体重200国际单位）预处理7天的动物，其磷脂与蛋白质比率的升高较低（两倍），Sph、PC和PE的变化也较小。当大鼠在同一时期用更高剂量的维生素E（每千克体重400国际单位）预处理时，细胞膜的脂质组成正常。这些结果表明，维生素E可以防止四氯化碳对肝细胞膜的诱导改变。维生素E对四氯化碳膜损伤的保护作用可能与其抗氧化特性有关。
