Barrett A D, Cubitt W D, Dimmock N J
J Gen Virol. 1984 Dec;65 ( Pt 12):2265-8. doi: 10.1099/0022-1317-65-12-2265.
By electron microscopy, particles of defective interfering Semliki Forest virus (DI SFV) had a mean diameter of 46.8 nm compared with 55.9 nm for standard virus particles, a decrease of 16%. The difference was confirmed by measurements of the two-dimensional projected areas of DI and standard virus particles. We examined nine different DI virus preparations produced by four to 13 undiluted passages in BHK cells and all were found to contain a majority of the smaller type of particle. Calculation of the absolute number of small particles showed that there were 130 particles per interfering unit measured by the inhibition of virus RNA synthesis. However, a more sensitive assay based on interference with virus protein synthesis gave a particle: interference ratio of 6.5.
通过电子显微镜观察，缺陷干扰性Semliki森林病毒（DI SFV）颗粒的平均直径为46.8纳米，而标准病毒颗粒的平均直径为55.9纳米，减少了16%。通过测量DI病毒颗粒和标准病毒颗粒的二维投影面积，证实了这种差异。我们检测了通过在BHK细胞中进行4至13次未稀释传代产生的9种不同的DI病毒制剂，发现它们都含有大多数较小类型的颗粒。对小颗粒绝对数量的计算表明，通过抑制病毒RNA合成测量，每干扰单位有130个颗粒。然而，基于干扰病毒蛋白质合成的更灵敏检测方法得出颗粒与干扰的比率为6.5。
