[Cytofluorometric analysis of cell kinetics in relation to tumor growth of advanced gastric cancers].

Correlated studies on both cell kinetics in association with nuclear ploidy pattern and pathological morphology of advanced gastric cancers were carried out using DNA-RNA cytofluorometry (NIKON SPM-RF1-D) with AO stain. The results showed that advanced gastric cancers could be divided into two main groups. Group 1 (12 cases) was the diploid cell population, commonly found in Borrmann type 4 showing diffuse infiltration of cancer cells, and group II (14 cases) was characterized by polyploidization, usually found in Borrmann type 2 or 3 showing adenocarcinomas composed of markedly pleomorphic cells. In addition, we further differentiated from group II four cases of cancers which included aneuploid cells with more pronounced pleomorphism than group II, and classified them as group II'. These results suggest that cell kinetics and nuclear ploidy pattern of the advanced gastric cancers are closely related to pathological characteristics, i.e., groups I and II to undifferentiated (gastric type) and differentiated (intestinal type) carcinomas, respectively.