Hirose K, Arai M, Nakagawara G
First Department of Surgery, Fukui Medical School, Japan.
Nihon Geka Gakkai Zasshi. 1991 Feb;92(2):122-6.
Expression of human epidermal growth factor (EGF) was examined immunohistologically in 93 surgically resected gastric carcinomas, using Biotin-StreptAvidin method pretreated by protease, and its relation with cancer progression and DNA ploidy pattern was studied. DNA ploidy pattern was determined by cytofluorometric measurement. The gastric cancers were divided into two basic ploidy patterns; a diploid mode and an aneuploid mode. EGF expressions were found in 5 out of 20 cases (25%) in intramucosal carcinomas and, the more deeply the carcinomas invaded into the gastric wall, the greater the frequency of EGF expressions did not always become. In the carcinomas with the aneuploid pattern, EGF expression was found only in 2 out of 12 cases (17%) in early cancers and significantly increased in advanced cancers, 15 out of 22 cases (68%). On the other hand, in the carcinomas with the diploid pattern, EGF was expressed in 9 out of 31 cases (29%) in advanced cancers, not significantly different in early cancers, 11 out of 28 cases (39%). Consequently, in advanced cancers, EGF was found more frequently in the aneuploid tumors than in the diploid tumors. These results suggested that EGF expression in the gastric carcinomas is closely correlated with the progression to the advanced cancer with DNA aneuploidy.
采用蛋白酶预处理的生物素-链霉亲和素法，对93例手术切除的胃癌进行免疫组织学检测，观察人表皮生长因子（EGF）的表达情况，并研究其与癌症进展及DNA倍体模式的关系。通过细胞荧光光度测定法确定DNA倍体模式。胃癌分为两种基本倍体模式：二倍体模式和非整倍体模式。在20例黏膜内癌中有5例（25%）发现EGF表达，且随着癌组织向胃壁浸润越深，EGF表达频率并非总是越高。在非整倍体模式的癌组织中，早期癌12例中有2例（17%）发现EGF表达，而在进展期癌中显著增加，22例中有15例（68%）。另一方面，在二倍体模式的癌组织中，进展期癌31例中有9例（29%）表达EGF，早期癌28例中有11例（39%）表达，两者无显著差异。因此，在进展期癌中，非整倍体肿瘤中EGF的表达比二倍体肿瘤更频繁。这些结果表明，胃癌中EGF的表达与伴有DNA非整倍体的进展期癌密切相关。
